Brd4 Assay Elisa

Description: ELISA

Description: ELISA

Description: ELISA

Description: ELISA

Description: ELISA

Description: ELISA

Description: A competitive ELISA for quantitative measurement of Canine True insulin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: Description of target: Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo . In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 4.68 pg/mL

Description: The 3x BRD TR-FRET Assay Buffer 2 is optimized for use with the following BPS Bioscience assay kits:_x000D_• TRIM24 TR-FRET Assay Kit #32630 384 reactions

Description: The BRDT (BD1 + BD2) TR-FRET Assay Kit is designed to measure the inhibition of_x000D_BRDT (BD1 + BD2) binding to its substrate in a homogeneous 384 reaction format. This_x000D_FRET-based assay requires no time-consuming washing steps, making it especially_x000D_suitable for high throughput screening applications. The assay procedure is_x000D_straightforward and simple; a sample containing terbium-labeled donor, dye-labeled_x000D_acceptor, BRDT, ligand, and an inhibitor is incubated for sixty minutes. Then, the_x000D_fluorescence intensity is measured using a fluorescence reader.

Description: Available in various conjugation types.