Comprehensive genomic analysis of microenvironment phenotypes in ovarian cancer

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Comprehensive genomic analysis of microenvironment phenotypes in ovarian cancer

Background: Ovarian most cancers is without doubt one of the main causes of cancer-related loss of life in girls. The incidence of ovarian most cancers is insidious, and the recurrence fee is excessive. The survival fee of ovarian most cancers has not considerably improved over the previous decade. Just lately, immune checkpoint inhibitors equivalent to these concentrating on CTLA-4, PD-1, or PD-L1 have been used to deal with ovarian most cancers. Due to this fact, a full evaluation of the immune biomarkers related to this malignancy is critical.
Strategies: On this research, we used information from The Most cancers Genome Atlas (TCGA) database to investigate the infiltration patterns of particular immune cell varieties in tumor samples. Knowledge from the Gene Expression Omnibus (GEO) database was used for exterior validation. In response to the invasion patterns of immune cells, we divided the ovarian most cancers microenvironment into two clusters: A and B.
These tumor microenvironment (TME) subtypes have been related to genomic and clinicopathological traits. Subsequently, a random forest classification mannequin was established. Differential genomic options, practical enrichment, and DNA methylation have been analyzed between the 2 clusters. The traits of immune cell infiltration and the expression of immune-related cytokines or markers have been analyzed. Somatic mutation evaluation was additionally carried out between clusters A and B. Lastly, multivariate Cox evaluation was used to investigate unbiased prognostic components.
Outcomes: The ovarian most cancers TME cluster A was characterised by much less infiltration of immune cells and sparse distribution and low expression of immunomodulators. In distinction, cytotoxic T cells and immunosuppressive cells have been considerably elevated within the ovarian most cancers TME cluster B. Moreover, immune-related cytokines or markers, together with IFN-γ and TNF-β, have been additionally expressed in giant portions.
In complete, 35 differentially methylated and expressed genes (DMEGs) have been recognized. Purposeful enrichment analyses revealed that the DMEGs in cluster B participated in vital organic processes and immune-related pathways. The mutation load in cluster B was insignificantly larger than that of cluster A (p = 0.076). Multivariate Cox evaluation confirmed that TME was an unbiased prognostic issue for ovarian most cancers (hazard ratio: 1.33, 95% confidence interval: 1.01-1.75, p = 0.041).
Conclusion: This research described and labeled fundamental details about the immune invasion sample of ovarian most cancers and built-in biomarkers associated to completely different immunophenotypes to disclose interactions between ovarian most cancers and the immune system.

Subsequent technology sequencing of SARS-CoV-2 genomes: challenges, functions and alternatives

Varied subsequent technology sequencing (NGS) primarily based methods have been efficiently used within the current previous for tracing origins and understanding the evolution of infectious brokers, investigating the unfold and transmission chains of outbreaks, in addition to facilitating the event of efficient and fast molecular diagnostic assessments and contributing to the hunt for remedies and vaccines.
The continuing COVID-19 pandemic poses one of many best international threats in trendy historical past and has already prompted extreme social and financial prices. The event of environment friendly and fast sequencing strategies to reconstruct the genomic sequence of SARS-CoV-2, the etiological agent of COVID-19, has been basic for the design of diagnostic molecular assessments and to plot efficient measures and methods to mitigate the diffusion of the pandemic. Numerous approaches and sequencing strategies can, as testified by the variety of out there sequences, be utilized to SARS-CoV-2 genomes. Nonetheless, every expertise and sequencing method has its personal benefits and limitations.
Within the present assessment, we’ll present a quick, however hopefully complete, account of at present out there platforms and methodological approaches for the sequencing of SARS-CoV-2 genomes. We additionally current a top level view of present repositories and databases that present entry to SARS-CoV-2 genomic information and related metadata. Lastly, we provide basic recommendation and pointers for the suitable sharing and deposition of SARS-CoV-2 information and metadata, and recommend that extra environment friendly and standardized integration of present and future SARS-CoV-2-related information would significantly facilitate the wrestle in opposition to this new pathogen. We hope that our ‘vademecum’ for the manufacturing and dealing with of SARS-CoV-2-related sequencing information, will contribute to this goal.
Comprehensive genomic analysis of microenvironment phenotypes in ovarian cancer
Comprehensive genomic analysis of microenvironment phenotypes in ovarian cancer

Transcriptional community modulated by the prognostic signature transcription components and their lengthy noncoding RNA companions in main prostate most cancers

Background: Transcriptional regulators are seminal gamers within the onset and development of prostate most cancers. Nonetheless, clarification of their underlying regulatory circuits and mechanisms calls for appreciable effort.
Strategies: Built-in analyses have been carried out on genomic, transcriptomic, and clinicopathological profiles of main prostate most cancers and transcription factor-binding profiles, which included estimating transcription issueexercise, figuring out transcription components of prognostic values, and discovering cis- and trans-regulations by lengthy noncoding RNAs. Interactions between transcription components and lengthy noncoding RNAs have been validated by RNA immunoprecipitation quantitative PCR. RNA interference assays have been carried out to discover roles of the chosen transcription regulators.
Findings: Sixteen transcription components, particularly ATOH1, have been collectively recognized as a prognostic signature. Candidate lengthy noncoding RNAs interplaying with the prognostic signature constituent transcription components have been additional found. Their interactions have been randomly checked, and lots of of them have been experimentally proved. Transcription regulation by MYC and its lengthy noncoding RNA companion AL590617.2 was additional validated on their candidate targets. Furthermore, the regulatory community ruled by the transcription components and their interacting lengthy noncoding RNA companions is illustrated and saved in our LNCTRN database

Pressure Injection Cell Clear - Mag

PC1000-MAG 1each
EUR 2272.8
Description: Capillary loader with transparent body for packing LC/MS columns and loading MS with integrated stir plate. 1000 psi max. includes a FRIT-KIT, hex wrench and 10 ferrules

Kinesis Rheodyne Injection valve PEEK with Switch - EACH

CHR2997 EACH
EUR 1765.8

Kinesis Rheodyne Preparative Injection Valve SS with Switch - EACH

CHR2487 EACH
EUR 2959.2

Swab Medical Pre Injection - PK100

SWA1000 PK100
EUR 10.13

Intravenous cannula (venflon) with injection port sterile various colours - PK50

393222 PK50
EUR 147.15

2ml Clear Type I Neutral Glass Injection Vial with 13mm Crimp Neck - PK344

VCDIN2R PK344
EUR 194.4

500 ML, CELL CULTURE GRADE WATER , WITH SEPTUM CAP; TESTED TO USP STERILE WATER FOR INJECTION SPECIFICATIONS

25-055-CVC 500 mL/pk
EUR 78
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

Vial Injection Stopper 20mm - PK1500

VIA1134 PK1500
EUR 373.95

Injection Stopper 20mm Butyl - PK1000

CHR2162 PK1000
EUR 199.8

Water for Injection(WFI), USP, Sterile

W0326-100 1 L
EUR 105.6

Water for Injection(WFI), USP, Sterile

W0326-202 20 L
EUR 699.6

Water for Injection(WFI), USP, Sterile

W0326-220 200 L
EUR 2652

Kinesis Rheodyne 7010 Injection Valve; SS - EACH

CHR2637 EACH
EUR 1387.8

Kinesis Injection Port Adapter; 10-32 - EACH

CHR3629 EACH
EUR 62.1

Kinesis Rheodyne Injection valve PEEK - EACH

CHR2993 EACH
EUR 1672.65

Grant TC120 Optima thermostat with integrated pump - EACH

BAT3924 EACH
EUR 1340.55

Grant TX150 Optima thermostat with integrated pump - EACH

BAT3930 EACH
EUR 1957.5

Grant TXF200 Optima thermostat with integrated pump - EACH

BAT3934 EACH
EUR 2502.9

Kinesis Rheodyne MXP Injection Valve; 6-Port; 2-Position - EACH

CHR3715 EACH
EUR 2582.55

Kinesis Rheodyne MXP Injection Valve; 10-Port; 2-Position - EACH

CHR3717 EACH
EUR 3576.15

Kinesis Rheodyne MXP Injection Valve; 6-Port; 2-Position; PEEK - EACH

CHR3721 EACH
EUR 2668.95

Stable Cas9 HEK293 Cell Line with Cas9 stably integrated at AAVS1 Safe Harbor Locus

CAS630A-1 2 x 10^6 cells
EUR 3142

A5 Integrated Hood & Masks - PK75

SAF4746 PK75
EUR 844.56

100L BENCH INTEGRATED TANK - EACH

ZBITANK01 EACH
EUR 2206.14

TPXScrewVial w integrated insert - PK1000

CHR2780 PK1000
EUR 1540.35

High Pressure Regulator

HPREG 1each
EUR 931.2
Description: High pressure regulator for use with PC77 pressure injection cells.

1mm Pressure Platen - RM

2398242 3unit
EUR 56.75

Two stage gas regulator for Helium or Nitrogen. 3000 psi maximum inlet pressure. 0-250 psi outlet pressure

105ABN4000 each
EUR 1130

Pressure transducer complete - EACH

WAT4820 EACH
EUR 1449.71

Bottle Pressure Plus 250mL - PK10

BOT8038 PK10
EUR 307.8

Bottle Pressure Plus 500mL - PK10

BOT8040 PK10
EUR 383.4

Kinesis Pressure Gauge Tee - EACH

CHR5011 EACH
EUR 163.35

In Line Pressure Regulator - EACH

INC6178 EACH
EUR 180.9

Sartolab P20 Pressure Filters - PK10

FIL6695 PK10
EUR 155.25

Flask Duran 1L Pressure Res. - PK10

2181054 PK10
EUR 271.35

funnel pressure equalising 50mL - EACH

FUN1322 EACH
EUR 136.35

funnel pressure equalising 100mL - EACH

FUN1324 EACH
EUR 145.8

funnel pressure equalising 250mL - EACH

FUN1326 EACH
EUR 180.9

funnel pressure equalising 500mL - EACH

FUN1328 EACH
EUR 217.35

Bottle Pressure Plusw/tSC 100mL - PK10

BOT8036 PK10
EUR 243

Pressure Plus 250mL Amber Bottle - PK10

BOT2272 PK10
EUR 556.2

Femtotip sterile injectioncapillary 0.5um - PK20

F5242952008 PK20
EUR 193.05

Femtotip II sterile injectioncapillary 0.5um - PK20

F5242957000 PK20
EUR 179.55

qPCR Pressure Sensi Adh SealRoll - 1ROLL

PCR0566 1ROLL
EUR 1266.3

Kinesis Pressure Relief Valve 100psi - EACH

CHR5031 EACH
EUR 241.65

IncuView Integrated Microscop Shelf, 45L (For H3565-45 Only)

H3565-45-LCI 1 each
EUR 4626.96

Kinesis MicroTight union ultraHigh Pressure 360um - EACH

CHR5123 EACH
EUR 193.05

Pressure Sealing Mats for 384 Well Format

GP504 5 mats
EUR 235.2

DURAN(R) PRESSURE PLUS BOTTLES VOLUME - PK10

Z742281-10EA PK10
EUR 274.05

XL Feed Pressure Regulator 0-25 bars - EACH

ZLXL000PR EACH
EUR 464.14

VentSep separatory funnel, with automatic pressure relief valve. PTFE stopcock, screw cap and liner. 1000ml

099ASST555 each
EUR 746

VentSep separatory funnel, with automatic pressure relief valve. PTFE stopcock, screw cap and liner. 2000ml

099ASST655 each
EUR 890

Spring Set - AE-6500(6450) - For Pressure Platen

2398283 8unit
EUR 17.69

1/4 NPTF Pressure Regulator w/ Gauge - EACH

ZSPRVBG01 EACH
EUR 508.2

Kinesis Pressure Relief Valve Tee; 1/16 - EACH

CHR4235 EACH
EUR 124.2

Kinesis Syringe; High Pressure 1ml 70mm 19G - EACH

CHR1609 EACH
EUR 383.4

Integrated Pre-heated 9kw boiler for Rodwell autoclaves for Rodwell autoclaves - EACH

AUT1379 EACH
EUR 5866.39

Kinesis Pressure Relief Valve Assembly 5 psi - EACH

CHR5029 EACH
EUR 241.65

Kinesis Low Pressure PEEK Tee; 1.0mm Thru-Hole - EACH

CHR4411 EACH
EUR 33.89

Kinesis MicroTight Tee ultraHigh Pressure 1/32 - EACH

CHR5133 EACH
EUR 368.55

Kimble NMR pressure caps PE for 5mm tubes red - PK100

TUB2602 PK100
EUR 33.75

Kinesis MicroTight Cross ultraHigh Pressure 1/32 - EACH

CHR5135 EACH
EUR 465.75

Kimble NMR pressure caps PE for 5mm tubes black - PK100

TUB2604 PK100
EUR 33.75

Kimble NMR pressure caps PE for 5mm tubes white - PK100

TUB2606 PK100
EUR 33.75

Kimble NMR pressure caps PE for 5mm tubes green - PK100

TUB2608 PK100
EUR 27

2 channel + pressure printer for Rodwell autoclaves - EACH

AUT1274 EACH
EUR 1926.45

Kimble NMR pressure caps PE for 5mm tubes yellow - PK100

TUB2610 PK100
EUR 33.75

IncuView Integrated Microscop Shelf, 180L (For H3565-180, 180HD and 180HDO2 Only)

H3565-180-LCI 1 each
EUR 4820.4

Factory fitted option Differential pressure gauge Minihelic - EACH

CAB1504 EACH
EUR 1128.04

Spare membrane filter for pressure equalization set 0.2 Ám - PK2

1129819 PK2
EUR 22.95

Kinesis PEEK Ferrule for High Pressure Fingertight (single) - EACH

CHR3363 EACH
EUR 8.1

Combination Air Filter/Pressure Regulator for Jack-O-Matic

108A2QD each
EUR 440

SLS Lab Pro S480 Digital Controller High Pressure Pump 50 Hz - EACH

SLS1035 EACH
EUR 5332.5

Set for pressure compensation for 4 port screw cap GL 18 GLS 80 - EACH

1160167 EACH
EUR 40.5

Pressure relief valve, for Glas-Col funnels SEP2000, or spout 12.5 - 13mm OD

099APRV10 each
EUR 205

FlowFAST V 12 Positive Pressure ISO Class III Laminar Flow Cabinet - EACH

CAB1424 EACH
EUR 12666.93

SEALING FILM, POLYESTER, 70 µM, PRESSURE SENSITIVE, ULTRA CLEAR, NONSTERILE, BULK

UC-500 100/pk
EUR 1093.2
Description: Sealing Products; Sealing films - Axygen

Pressure relief valve, for Glas-Col funnels SEP0060 - SEP1000, or spout 7.5 - 9.5mm OD

099APRV6 each
EUR 205

Duran GL45 500ML Clear Lab Glass Bottle Pressure Plus No Cap or Pouring Ring - PK10

1092235 PK10
EUR 236.25

Set for pressure compensation 2 and 3 port screw cap incl. 0.2 ┴m membrane filter - EACH

1137799 EACH
EUR 40.5

Kinesis Crimp Cap Headspace 20mm; Silicone White/PTFE Beige Septa (Pressure Release) - PK1000

CHR1172 PK1000
EUR 945

Sealing film, qPCR Optical (pressure-sensitive adhesive) ABI Type, 10 bags of 10 seals, 100/pk

P1001-QPS 100/pack
EUR 165.8
Description: Sealing film qPCR Optical (pressure-sensitive adhesive) ABI Type

SLS Lab Pro S480 Recirculating Chiller Basic Version 2 Litre High Pressure Pump 50Hz - EACH

SLS1007 EACH
EUR 4456.35

SLS Lab Pro S480 Recirculating Chiller Basic Version 3.5 Litre High Pressure Pump 50Hz - EACH

SLS1011 EACH
EUR 4374

1-Step Polymorphs, Human Cell Separation

71-AN221725 1
EUR 238.8

Kinesis RheFlex High Pressure SS Fitting set for 1/8 OD Tubing 5/16-24 Coned for 1/8 OD Tubing - EACH

CHR2513 EACH
EUR 31.05

SLS Lab Pro S500 Mini Recirculating Chiller touchscreen Controller 3.5L/min low Pressure Pump 90-230V 50/60Hz - EACH

SLS1047 EACH
EUR 6318

GMP Recombinant Human Interleukin-4 (IL-4)

GMPhuIL-4-50ug 50ug
EUR 528

PRE-GMP rHu GM-CSF, Molgramostim-Leukoma

04-RHUGM-CSF-7A10 300 µg
EUR 462
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.

Kinesis Rheodyne 7725 Sample Injector SS with Switch - EACH

CHR2785 EACH
EUR 1773.9

Gentodenz

19-DENZ-500 500 g
EUR 416

Silver crimp headspace cap (20mm) (Aluminium: white silicone/beige PTFE septa: 3.0mm thickness) - pressure release ; Level 2 High-throughput Applications - PK100

6APRCC20-ST1 PK100
EUR 64.8

Silver crimp headspace cap (20mm) (Aluminium: white silicone/beige PTFE septa: 3.0mm thickness) - pressure release ; Level 2 High-throughput Applications - PK5000

6APRCC20-ST1F PK5000
EUR 2899.8

Silver crimp headspace cap (20mm) (Aluminium: white silicone/beige PTFE septa: 3.0mm thickness) - pressure release ; Level 2 High-throughput Applications - PK1000

6APRCC20-ST1T PK1000
EUR 611.55

Fluoro IndoBlu (with 5 Black Opaque Plates)

INDBLU100-3P 500 Tests
EUR 172

Fluoro IndoBlu (with 10 Black Opaque Plates)

INDBLU100-4P 1000 Tests
EUR 322.5

aCella - TOX 500 Tests (with 5 Lumi Plates)

CLATOX100-3L 500 Tests
EUR 910

5X RIPA Buffer with Protease Inhibitor Cocktail

AKR-190 20mL
EUR 100

Rye Agar A

765-M1854-500G 500 g
EUR 82

Rye Agar B

765-M1855-500G 500 g
EUR 93

Porcine Parvovirus Antibody Elisa Test Kit

767-LSY-30009 192 Wells/kit
EUR 382

QuantiChrom Hemoglobin Assay Kit

65-DIHB-250 250 tests
EUR 473

Malachite Green Phosphate Assay kit

65-POMG-25H 2500 tests
EUR 333

50ml TC Tubes, Conical, 440 units/box

04-5540150 440 units/box
EUR 85.2

GMP IL4, 50µg

04-GMP-HU-IL4-50UG 50µg
EUR 579.6
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE

04-GSB
  • Ask for price
  • Ask for price
  • 1L
  • 500mL
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You!

rHu IL 2 , 3MIU

04-RHIL2-02F01 1 vial
EUR 298.8
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

rHu IL 2 , 3MIU , Lot 200908F02

04-RHIL2-08F02 1 vial
EUR 298.8
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

Mouse Anti TNP Immunotoxicity

198-TNPG-1 100 µL
EUR 469.2

Exo-Check Exosome Antibody Array

322-EXO-FBS-50A-1 100 µg
EUR 469.2

Monkeypox Virus Real Time PCR Kit

ZD-0076-01 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit

ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

EasyNat Quattro

UC0104 4-module system
EUR 12000

EasyNat Octo

UC0108 8-module system
EUR 22000

Kinesis Siltite Base Seal; Split; Splitless Injector; compatible with Agilent GC - PK2

CHR1909 PK2
EUR 157.95

Kinesis Siltite Base Seal; Split; Splitless Injector; compatible with Agilent GC - PK10

CHR1911 PK10
EUR 626.4

aCella - TOX 500 Tests (with 5 Lumi Plates + 5 TC Plates)

CLATOX100-3P 500 Tests
EUR 965

CytoSelect Clonogenic Tumor Cell Isolation Kit (5 preps)

CBA-155 5 preps
EUR 957.6
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

CytoSelect Clonogenic Tumor Cell Isolation Kit (5 x 5 preps)

CBA-155-5 25 preps
EUR 3894
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

293AD Cell Line

AD-100 1 vial
EUR 365

293AAV Cell Line

AAV-100 1 vial
EUR 405

293LTV Cell Line

LTV-100 1 vial
EUR 405

293RTV Cell Line

RV-100 1 vial
EUR 405

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325 96 assays
EUR 1027.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325-5 5 x 96 assays
EUR 4033.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135 96 assays
EUR 715

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135-5 5 x 96 assays
EUR 3095

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140 96 assays
EUR 750

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140-5 5 x 96 assays
EUR 3215

293/GFP Cell Line

AKR-200 1 vial
EUR 460

T47D/GFP Cell Line

AKR-208 1 vial
EUR 686.4
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 460

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 460

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 460

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 460

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 460

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 460

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size

CBA-135-T 24 assays
EUR 518.4
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

MCF-7/Luc Cell Line

AKR-234 1 vial
EUR 686.4
Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 686.4
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size

CBA-140-T 24 assays
EUR 547.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

Collagen-based Cell Contraction Assay

CBA-201 24 assays
EUR 420

Radius 384-Well Cell Migration Assay

CBA-127 384 assays
EUR 721.2
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 384-Well Cell Migration Assay

CBA-127-5 5 x 384 wells
EUR 2802
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

HIF-1 Alpha Cell Based ELISA Kit

CBA-281 96 assays
EUR 734.4
Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat.

CytoSelect™ MTT Cell Proliferation Assay

CBA-252 960 assays
EUR 320

Radius™ 24-Well Cell Migration Assay

CBA-125 24 assays
EUR 425

Radius™ 24-Well Cell Migration Assay

CBA-125-5 5 x 24 assays
EUR 1820

Radius™ 96-Well Cell Migration Assay

CBA-126 96 assays
EUR 495

Radius™ 96-Well Cell Migration Assay

CBA-126-5 5 x 96 assays
EUR 2095

Radius™ 48-Well Cell Migration Assay

CBA-5037 48 assays
EUR 445

Radius™ 48-Well Cell Migration Assay

CBA-5037-5 5 x 48 assays
EUR 1895

CytoSelect™ BrdU Cell Proliferation ELISA Kit

CBA-251 96 assays
EUR 455

CytoSelect Cell Proliferation Assay Reagent (Fluorometric)

CBA-250 10 mL
EUR 490.8
Description: Cell Biolabs? CytoSelect Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then incubated with the proliferation reagent.  Upon entering metabolically active live cells, the non-fluorescent proliferation reagent is converted into a bright red fluorescent form. An increase in cell proliferation is accompanied by increased fluorescent signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.

CytoSelect™ 96-well Cell Transformation Assay

CBA-130 96 assays
EUR 640

CytoSelect™ 96-well Cell Transformation Assay

CBA-130-5 5 x 96 assays
EUR 2695
Interpretation: The prognostic signature constituent transcription components and their interacting lengthy noncoding RNAs could characterize promising biomarkers and/or therapeutic targets for prostate most cancers. Moreover, the computational framework proposed within the current research may be utilized to discover essential transcriptional regulators in different varieties of most cancers.

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