Comprehensive genomic analysis of microenvironment phenotypes in ovarian cancer

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Comprehensive genomic analysis of microenvironment phenotypes in ovarian cancer

Background: Ovarian most cancers is without doubt one of the main causes of cancer-related loss of life in girls. The incidence of ovarian most cancers is insidious, and the recurrence fee is excessive. The survival fee of ovarian most cancers has not considerably improved over the previous decade. Just lately, immune checkpoint inhibitors equivalent to these concentrating on CTLA-4, PD-1, or PD-L1 have been used to deal with ovarian most cancers. Due to this fact, a full evaluation of the immune biomarkers related to this malignancy is critical.
Strategies: On this research, we used information from The Most cancers Genome Atlas (TCGA) database to investigate the infiltration patterns of particular immune cell varieties in tumor samples. Knowledge from the Gene Expression Omnibus (GEO) database was used for exterior validation. In response to the invasion patterns of immune cells, we divided the ovarian most cancers microenvironment into two clusters: A and B.
These tumor microenvironment (TME) subtypes have been related to genomic and clinicopathological traits. Subsequently, a random forest classification mannequin was established. Differential genomic options, practical enrichment, and DNA methylation have been analyzed between the 2 clusters. The traits of immune cell infiltration and the expression of immune-related cytokines or markers have been analyzed. Somatic mutation evaluation was additionally carried out between clusters A and B. Lastly, multivariate Cox evaluation was used to investigate unbiased prognostic components.
Outcomes: The ovarian most cancers TME cluster A was characterised by much less infiltration of immune cells and sparse distribution and low expression of immunomodulators. In distinction, cytotoxic T cells and immunosuppressive cells have been considerably elevated within the ovarian most cancers TME cluster B. Moreover, immune-related cytokines or markers, together with IFN-γ and TNF-β, have been additionally expressed in giant portions.
In complete, 35 differentially methylated and expressed genes (DMEGs) have been recognized. Purposeful enrichment analyses revealed that the DMEGs in cluster B participated in vital organic processes and immune-related pathways. The mutation load in cluster B was insignificantly larger than that of cluster A (p = 0.076). Multivariate Cox evaluation confirmed that TME was an unbiased prognostic issue for ovarian most cancers (hazard ratio: 1.33, 95% confidence interval: 1.01-1.75, p = 0.041).
Conclusion: This research described and labeled fundamental details about the immune invasion sample of ovarian most cancers and built-in biomarkers associated to completely different immunophenotypes to disclose interactions between ovarian most cancers and the immune system.

Subsequent technology sequencing of SARS-CoV-2 genomes: challenges, functions and alternatives

Varied subsequent technology sequencing (NGS) primarily based methods have been efficiently used within the current previous for tracing origins and understanding the evolution of infectious brokers, investigating the unfold and transmission chains of outbreaks, in addition to facilitating the event of efficient and fast molecular diagnostic assessments and contributing to the hunt for remedies and vaccines.
The continuing COVID-19 pandemic poses one of many best international threats in trendy historical past and has already prompted extreme social and financial prices. The event of environment friendly and fast sequencing strategies to reconstruct the genomic sequence of SARS-CoV-2, the etiological agent of COVID-19, has been basic for the design of diagnostic molecular assessments and to plot efficient measures and methods to mitigate the diffusion of the pandemic. Numerous approaches and sequencing strategies can, as testified by the variety of out there sequences, be utilized to SARS-CoV-2 genomes. Nonetheless, every expertise and sequencing method has its personal benefits and limitations.
Within the present assessment, we’ll present a quick, however hopefully complete, account of at present out there platforms and methodological approaches for the sequencing of SARS-CoV-2 genomes. We additionally current a top level view of present repositories and databases that present entry to SARS-CoV-2 genomic information and related metadata. Lastly, we provide basic recommendation and pointers for the suitable sharing and deposition of SARS-CoV-2 information and metadata, and recommend that extra environment friendly and standardized integration of present and future SARS-CoV-2-related information would significantly facilitate the wrestle in opposition to this new pathogen. We hope that our ‘vademecum’ for the manufacturing and dealing with of SARS-CoV-2-related sequencing information, will contribute to this goal.
Comprehensive genomic analysis of microenvironment phenotypes in ovarian cancer
Comprehensive genomic analysis of microenvironment phenotypes in ovarian cancer

Transcriptional community modulated by the prognostic signature transcription components and their lengthy noncoding RNA companions in main prostate most cancers

Background: Transcriptional regulators are seminal gamers within the onset and development of prostate most cancers. Nonetheless, clarification of their underlying regulatory circuits and mechanisms calls for appreciable effort.
Strategies: Built-in analyses have been carried out on genomic, transcriptomic, and clinicopathological profiles of main prostate most cancers and transcription factor-binding profiles, which included estimating transcription issueexercise, figuring out transcription components of prognostic values, and discovering cis- and trans-regulations by lengthy noncoding RNAs. Interactions between transcription components and lengthy noncoding RNAs have been validated by RNA immunoprecipitation quantitative PCR. RNA interference assays have been carried out to discover roles of the chosen transcription regulators.
Findings: Sixteen transcription components, particularly ATOH1, have been collectively recognized as a prognostic signature. Candidate lengthy noncoding RNAs interplaying with the prognostic signature constituent transcription components have been additional found. Their interactions have been randomly checked, and lots of of them have been experimentally proved. Transcription regulation by MYC and its lengthy noncoding RNA companion AL590617.2 was additional validated on their candidate targets. Furthermore, the regulatory community ruled by the transcription components and their interacting lengthy noncoding RNA companions is illustrated and saved in our LNCTRN database

Pressure Injection Cell Clear

PC1000 1each
EUR 1705.2
Description: Capillary loader with transparent body for packing LC/MS columns and loading MS. 1000 psi max. includes a FRIT-KIT, hex wrench and 10 ferrules

Pressure Injection Cell Clear - Mag

PC1000-MAG 1each
EUR 2272.8
Description: Capillary loader with transparent body for packing LC/MS columns and loading MS with integrated stir plate. 1000 psi max. includes a FRIT-KIT, hex wrench and 10 ferrules

Kinesis Rheodyne Injection valve PEEK with Switch

CHR2997 EACH
EUR 1569.6

Kinesis Rheodyne Preparative Injection Valve SS with Switch

CHR2487 EACH
EUR 2630.4

Swab Medical Pre Injection

SWA1000 PK100
EUR 9.01

Intravenous cannula (venflon) with injection port sterile various colours

393222 PK50
EUR 130.8

Vial Injection Stopper 20mm

VIA1134 PK1500
EUR 332.4

Injection Stopper 20mm Butyl

CHR2162 PK1000
EUR 177.6

2ml Clear Type I Neutral Glass Injection Vial with 13mm Crimp Neck

VCDIN2R PK344
EUR 172.8

Water for Injection(WFI), USP, Sterile

W0326-100 1 L
EUR 105.6

Water for Injection(WFI), USP, Sterile

W0326-202 20 L
EUR 699.6

Water for Injection(WFI), USP, Sterile

W0326-220 200 L
EUR 2652

500 ML, CELL CULTURE GRADE WATER , WITH SEPTUM CAP; TESTED TO USP STERILE WATER FOR INJECTION SPECIFICATIONS

25-055-CVC 500 mL/pk
EUR 78
Description: Media Catalog; Sterile Wi-Fi Qual, Cell

Kinesis Rheodyne 7010 Injection Valve; SS

CHR2637 EACH
EUR 1233.6

Kinesis Rheodyne Injection valve PEEK

CHR2993 EACH
EUR 1486.8

Kinesis Injection Port Adapter; 10-32

CHR3629 EACH
EUR 55.2

Grant TC120 Optima thermostat with integrated pump

BAT3924 EACH
EUR 1191.6

Grant TX150 Optima thermostat with integrated pump

BAT3930 EACH
EUR 1740

Grant TXF200 Optima thermostat with integrated pump

BAT3934 EACH
EUR 2224.8

Kinesis Rheodyne MXP Injection Valve; 6-Port; 2-Position

CHR3715 EACH
EUR 2295.6

Kinesis Rheodyne MXP Injection Valve; 10-Port; 2-Position

CHR3717 EACH
EUR 3178.8

Stable Cas9 HEK293 Cell Line with Cas9 stably integrated at AAVS1 Safe Harbor Locus

CAS630A-1 2 x 10^6 cells
EUR 3142

Kinesis Rheodyne MXP Injection Valve; 6-Port; 2-Position; PEEK

CHR3721 EACH
EUR 2372.4

A5 Integrated Hood & Masks

SAF4746 PK75
EUR 750.72

100L BENCH INTEGRATED TANK

ZBITANK01 EACH
EUR 1961.01

TPXScrewVial w integrated insert

CHR2780 PK1000
EUR 1369.2

High Pressure Regulator

HPREG 1each
EUR 931.2
Description: High pressure regulator for use with PC77 pressure injection cells.

1mm Pressure Platen - RM

2398242 3unit
EUR 337.2

Pressure transducer complete

WAT4820 EACH
EUR 1288.63

Bottle Pressure Plus 250mL

BOT8038 PK10
EUR 273.6

Bottle Pressure Plus 500mL

BOT8040 PK10
EUR 340.8

Kinesis Pressure Gauge Tee

CHR5011 EACH
EUR 145.2

In Line Pressure Regulator

INC6178 EACH
EUR 160.8

Sartolab P20 Pressure Filters

FIL6695 PK10
EUR 138

Flask Duran 1L Pressure Res.

2181054 PK10
EUR 241.2

funnel pressure equalising 50mL

FUN1322 EACH
EUR 121.2

funnel pressure equalising 100mL

FUN1324 EACH
EUR 129.6

funnel pressure equalising 250mL

FUN1326 EACH
EUR 160.8

funnel pressure equalising 500mL

FUN1328 EACH
EUR 193.2

Bottle Pressure Plusw/tSC 100mL

BOT8036 PK10
EUR 216

Pressure Plus 250mL Amber Bottle

BOT2272 PK10
EUR 494.4

qPCR Pressure Sensi Adh SealRoll

PCR0566 1ROLL
EUR 1125.6

Kinesis Pressure Relief Valve 100psi

CHR5031 EACH
EUR 214.8

Kinesis MicroTight union ultraHigh Pressure 360um

CHR5123 EACH
EUR 171.6

DURAN(R) PRESSURE PLUS BOTTLES VOLUME

Z742281-10EA PK10
EUR 243.6

XL Feed Pressure Regulator 0-25 bars

ZLXL000PR EACH
EUR 412.57

IncuView Integrated Microscop Shelf, 45L (For H3565-45 Only)

H3565-45-LCI 1 each
EUR 1884.6

1/4 NPTF Pressure Regulator w/ Gauge

ZSPRVBG01 EACH
EUR 451.74

Femtotip sterile injectioncapillary 0.5um

F5242952008 PK20
EUR 171.6

Femtotip II sterile injectioncapillary 0.5um

F5242957000 PK20
EUR 159.6

Kinesis Pressure Relief Valve Tee; 1/16

CHR4235 EACH
EUR 110.4

Pressure Sealing Mats for 384 Well Format

GP504 5 mats
EUR 235.2

Kinesis Syringe; High Pressure 1ml 70mm 19G

CHR1609 EACH
EUR 340.8

Spring Set - AE-6500(6450) - For Pressure Platen

2398283 8unit
EUR 300

Integrated Pre-heated 9kw boiler for Rodwell autoclaves for Rodwell autoclaves

AUT1379 EACH
EUR 5214.57

Kinesis Pressure Relief Valve Assembly 5 psi

CHR5029 EACH
EUR 214.8

Kinesis Low Pressure PEEK Tee; 1.0mm Thru-Hole

CHR4411 EACH
EUR 30.12

Kimble NMR pressure caps PE for 5mm tubes red

TUB2602 PK100
EUR 30

Kinesis MicroTight Tee ultraHigh Pressure 1/32

CHR5133 EACH
EUR 327.6

Kimble NMR pressure caps PE for 5mm tubes black

TUB2604 PK100
EUR 30

Kimble NMR pressure caps PE for 5mm tubes white

TUB2606 PK100
EUR 30

Kimble NMR pressure caps PE for 5mm tubes green

TUB2608 PK100
EUR 24

Kinesis MicroTight Cross ultraHigh Pressure 1/32

CHR5135 EACH
EUR 414

Kimble NMR pressure caps PE for 5mm tubes yellow

TUB2610 PK100
EUR 30

2 channel + pressure printer for Rodwell autoclaves

AUT1274 EACH
EUR 1712.4

IncuView Integrated Microscop Shelf, 180L (For H3565-180, 180HD and 180HDO2 Only)

H3565-180-LCI 1 each
EUR 1980.9

Kinesis PEEK Ferrule for High Pressure Fingertight (single)

CHR3363 EACH
EUR 7.2

Factory fitted option Differential pressure gauge Minihelic

CAB1504 EACH
EUR 1002.7

Spare membrane filter for pressure equalization set 0.2 Ám

1129819 PK2
EUR 20.4

SLS Lab Pro S480 Digital Controller High Pressure Pump 50 Hz

SLS1035 EACH
EUR 4740

Set for pressure compensation for 4 port screw cap GL 18 GLS 80

1160167 EACH
EUR 36

FlowFAST V 12 Positive Pressure ISO Class III Laminar Flow Cabinet

CAB1424 EACH
EUR 11259.49

Sealing film, qPCR Optical (pressure-sensitive adhesive) ABI Type, 100/pk

P1001-QPS 100/pack
EUR 192.84
Description: Sealing film qPCR Optical (pressure-sensitive adhesive) ABI Type

SEALING FILM, POLYESTER, 70 µM, PRESSURE SENSITIVE, ULTRA CLEAR, NONSTERILE, BULK

UC-500 100/pk
EUR 1093.2
Description: Sealing Products; Sealing films - Axygen

Duran GL45 500ML Clear Lab Glass Bottle Pressure Plus No Cap or Pouring Ring

1092235 PK10
EUR 210

Set for pressure compensation 2 and 3 port screw cap incl. 0.2 ┴m membrane filter

1137799 EACH
EUR 36

Kinesis Crimp Cap Headspace 20mm; Silicone White/PTFE Beige Septa (Pressure Release)

CHR1172 PK1000
EUR 840

SLS Lab Pro S480 Recirculating Chiller Basic Version 2 Litre High Pressure Pump 50Hz

SLS1007 EACH
EUR 3961.2

SLS Lab Pro S480 Recirculating Chiller Basic Version 3.5 Litre High Pressure Pump 50Hz

SLS1011 EACH
EUR 3888

Kinesis RheFlex High Pressure SS Fitting set for 1/8 OD Tubing 5/16-24 Coned for 1/8 OD Tubing

CHR2513 EACH
EUR 27.6

SLS Lab Pro S500 Mini Recirculating Chiller touchscreen Controller 3.5L/min low Pressure Pump 90-230V 50/60Hz

SLS1047 EACH
EUR 5616

1-Step Polymorphs, Human Cell Separation

71-AN221725 1
EUR 238.8

Kinesis Rheodyne 7725 Sample Injector SS with Switch

CHR2785 EACH
EUR 1576.8

Silver crimp headspace cap (20mm) (Aluminium: white silicone/beige PTFE septa: 3.0mm thickness) - pressure release ; Level 2 High-throughput Applications

6APRCC20-ST1 PK100
EUR 57.6

Silver crimp headspace cap (20mm) (Aluminium: white silicone/beige PTFE septa: 3.0mm thickness) - pressure release ; Level 2 High-throughput Applications

6APRCC20-ST1F PK5000
EUR 2577.6

Silver crimp headspace cap (20mm) (Aluminium: white silicone/beige PTFE septa: 3.0mm thickness) - pressure release ; Level 2 High-throughput Applications

6APRCC20-ST1T PK1000
EUR 543.6

GMP Recombinant Human Interleukin-4 (IL-4)

GMPhuIL-4-50ug 50ug
EUR 528

Gentodenz

19-DENZ-500 500 g
EUR 416

PRE-GMP rHu GM-CSF, Molgramostim-Leukoma

04-RHUGM-CSF-7A10 300 µg
EUR 462
Description: Recombinant human GM-CSF produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.5kD.

Kinesis Siltite Base Seal; Split; Splitless Injector; compatible with Agilent GC

CHR1909 PK2
EUR 140.4

Kinesis Siltite Base Seal; Split; Splitless Injector; compatible with Agilent GC

CHR1911 PK10
EUR 556.8

5X RIPA Buffer with Protease Inhibitor Cocktail

AKR-190 20mL
EUR 192
Description: RIPA Buffer extracts proteins from cultured mammalian cells for various downstream applications.

50ml TC Tubes, Conical, 440 units/box

04-5540150 440 units/box
EUR 85.2

GMP IL4, 50µg

04-GMP-HU-IL4-50UG 50µg
EUR 579.6
Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE

04-GSB
  • EUR 144.00
  • EUR 96.00
  • 1L
  • 500mL
Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You!

rHu IL 2 , 3MIU

04-RHIL2-02F01 1 vial
EUR 298.8
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

rHu IL 2 , 3MIU , Lot 200908F02

04-RHIL2-08F02 1 vial
EUR 298.8
Description: Recombinant human interleukin-2 is a sterile protein product for injection. rHuIL-2 is produced by recombinant DNA technology using Yeast. It is a highly purified protein containing 133 amino acids, with cysteine mutated to alanine at 125 amino acid position, and has a molecular weight of approximately 15.4kD, non-glycosylated.

Mouse Anti TNP Immunotoxicity

198-TNPG-1 100 µL
EUR 469.2

Rye Agar A

765-M1854-500G 500 g
EUR 82

Rye Agar B

765-M1855-500G 500 g
EUR 93

Porcine Parvovirus Antibody Elisa Test Kit

767-LSY-30009 192 Wells/kit
EUR 382

QuantiChrom Hemoglobin Assay Kit

65-DIHB-250 250 tests
EUR 473

Malachite Green Phosphate Assay kit

65-POMG-25H 2500 tests
EUR 333

Exo-Check Exosome Antibody Array

322-EXO-FBS-50A-1 100 µg
EUR 469.2

EasyNat Quattro

UC0104 4-module system
EUR 12000

EasyNat Octo

UC0108 8-module system
EUR 22000

Monkeypox Virus Real Time PCR Kit

ZD-0076-01 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit

ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

CytoSelect Clonogenic Tumor Cell Isolation Kit (5 preps)

CBA-155 5 preps
EUR 957.6
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

CytoSelect Clonogenic Tumor Cell Isolation Kit (5 x 5 preps)

CBA-155-5 25 preps
EUR 3894
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

293AD Cell Line

AD-100 1 vial
EUR 553.2
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 609.6
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 609.6
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293RTV Cell Line

RV-100 1 vial
EUR 609.6
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135 96 assays
EUR 985.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135-5 5 x 96 assays
EUR 4027.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140 96 assays
EUR 1027.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140-5 5 x 96 assays
EUR 4179.6
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325 96 assays
EUR 1027.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325-5 5 x 96 assays
EUR 4033.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

293/GFP Cell Line

AKR-200 1 vial
EUR 686.4
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line

AKR-208 1 vial
EUR 686.4
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 686.4
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 686.4
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 686.4

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 686.4

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 686.4
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

Injector Unit 16 Jets

WAS6106 EACH
EUR 2155.55

Injector Unit 19 Jets

WAS6108 EACH
EUR 1510.58

Injector Unit 39 Jets

WAS6116 EACH
EUR 2115.07

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 686.4

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size

CBA-135-T 24 assays
EUR 518.4
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

Injector Jet 2.5x90mm

WAS6146 EACH
EUR 47

MCF-7/Luc Cell Line

AKR-234 1 vial
EUR 686.4
Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 686.4
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

Injector Jet 4x160mm

WAS6138 EACH
EUR 30.03

Injector Jet 6x200mm

WAS6156 EACH
EUR 47

Injector Jet 6x220mm

WAS6158 EACH
EUR 47

Injector Jet 6x240mm

WAS6160 EACH
EUR 47

Basket 48 injectors for LX/LXP

WAS2320 EACH
EUR 2494.8

CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size

CBA-140-T 24 assays
EUR 547.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

Basket for the con two injector

WAS7208 EACH
EUR 631.91

Collagen-based Cell Contraction Assay

CBA-201 24 assays
EUR 582
Description: Cell Biolabs? Collagen-based Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators. Each kit provides sufficient quantities to perform up to 24 assays in a 24-well plate. The kit can be also used in culturing cells in 3D collagen matrix.

CytoSelect MTT Cell Proliferation Assay

CBA-252 960 assays
EUR 490.8
Description: Cell Biolabs? CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation.  The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1).  An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

Radius 24-Well Cell Migration Assay

CBA-125 24 assays
EUR 602.4
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 24-Well Cell Migration Assay

CBA-125-5 5 x 24 assays
EUR 2362.8
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay

CBA-126 96 assays
EUR 686.4
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay

CBA-126-5 5 x 96 assays
EUR 2697.6
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 384-Well Cell Migration Assay

CBA-127 384 assays
EUR 721.2
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 384-Well Cell Migration Assay

CBA-127-5 5 x 384 wells
EUR 2802
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 48-Well Cell Migration Assay

CBA-5037 48 assays
EUR 622.8
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 48-Well Cell Migration Assay

CBA-5037-5 5 x 48 assays
EUR 2454
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Basket 64 Injectors Lancer

WAS2318 EACH
EUR 2514

HIF-1 Alpha Cell Based ELISA Kit

CBA-281 96 assays
EUR 734.4
Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat.

CytoSelect BrdU Cell Proliferation ELISA Kit

CBA-251 96 assays
EUR 637.2
Description: The CytoSelect BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody.  When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells.  Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection).  Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU.  The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.

CytoSelect 96-well Cell Transformation Assay

CBA-130 96 assays
EUR 866.4
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.
Interpretation: The prognostic signature constituent transcription components and their interacting lengthy noncoding RNAs could characterize promising biomarkers and/or therapeutic targets for prostate most cancers. Moreover, the computational framework proposed within the current research may be utilized to discover essential transcriptional regulators in different varieties of most cancers.

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