In vivo MRS measurement of 2-hydroxyglutarate in patient-derived IDH-mutant xenograft mouse models versus glioma patients.

To produce a preclinical model isocitrate dehydrogenase (IDH) of the mutant glioma glioma patients and devise methods to test the compatibility MRS 2-hydroxyglutarate production (2HG) between preclinical models and patients.

Five patient-derived xenograft (PDX) rat glioma generated from two patients with IDH1 R132H mutation. A sequence PRESS adjusted at 9.4 T, with a phantom computer simulation and analysis, to improve the detection of 2HG in mice. 2HG and other metabolites in rats was measured using MRS optimized PDX at 9.4 T and compared with 3 T MRS measurements of metabolites in parent-tumor patients.

LCModel spectral fitting is done by using the home as a spectrum. metabolite levels were measured with reference to water.The PRESS TE optimized to 96 ms, which 2HG 2.25 ppm signal is narrow and overturned, causing a strict separation of resonance 2HG of adjacent signals from other metabolites. MRS optimized supplied precise detection 2HG in mice compared to short-TE MRS at 9.4 T. estimate of 2HG in PDX rats in good agreement with measurements of 2HG in 2HG production patients.The similarity between the model and parent PDX-tumor patients showed that the tumor PDX IDH maintain metabolic fingerprint parents and can serve as a preclinical model to improve our understanding of IDH mutations associated metabolic reprogramming.

Mutations start contributing to the development of cancer is sometimes present in premalignant cells. Is this mutation targeting therapy can eradicate premalignant cells is unclear. Acute myeloid leukemia (AML) is an interesting system to investigate the effects of preventive treatment for the disease is often preceded by premalignant state (clonal hematopoiesis or myelodysplastic syndromes).

In Npm1c / Dnmt3a mutant knock-in mice, the model of development of AML, leukemia preceded by an extended period of myeloid progenitor cell proliferation and self-renewal. We discovered that this self-renewal can be reversed with oral administration of small molecule (VTP-50 469) that targets MLL1-Menin chromatin complexes. The results of the preclinical support the hypothesis that high-risk individuals develop AML may benefit from targeted epigenetic therapy in the prevention setting.

The acquisition of genetic mutations in a group A streptococcus at the site of infection and subsequent systemic spread of the mutant with a lethal mutation in a mouse model of streptococcal toxic shock syndrome.

Streptococcal toxic shock syndrome (STSS) is caused mainly by Streptococcus pyogenes (Group A Streptococcus, GAS), and has a mortality rate of 25%. Mutations in CsrRS and RopB, which suppresses the transcription of many virulence factors, was recently found in clinical isolates from STSS patients, but do not fully understand when and where GAS acquire a mutation in the host.

To resolve this question, we use our mouse models of human STSS GAS strains to recover from the injection sites, spleen and blood of mice dying with STSS-like symptoms, and analyzing sequence covR / covS genes and genes encoding ropB CsrRS and RopB. Fifteen of the twenty mice were inoculated transdermally into the muscle with GAS organisms become moribund with STSS-like symptoms after more than 20 days after the inoculation.

cDNA - Human Adult Normal Tissue: Skin
C1234218	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Brain
C1234035	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Colon
C1234090	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Heart
C1234122	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Ovary
C1234183	Biochain	40 reactions	EUR 1049

cDNA - Human Adult Normal Tissue: Theca
C1234261-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Breast
C1234086	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Kidney
C1234142	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Rectum
C1234206	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Spleen
C1234246	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Testis
C1234260	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Throat
C1234263-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Thymus
C1234264	Biochain	40 reactions	EUR 1049

cDNA - Human Adult Normal Tissue: Tongue
C1234267-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Tonsil
C1234268-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Uterus
C1234274	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Adipose
C1234003-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Adrenal
C1234004	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Bladder
C1234010	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Trachea
C1234160-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Stomach
C1234248	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Thyroid
C1234265	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Pancreas
C1234188	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Placenta
C1234200	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Prostate
C1234201	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Esophagus
C1234106	Biochain	40 reactions	EUR 385

Frozen Tissue Section - Human Adult Normal: Liver
T1234149	Biochain	5 slides	EUR 343

Total RNA - Human Adult Normal Tissue: Liver
R1234149-50	Biochain	50 ug	EUR 170

Paraffin Tissue Section - Human Adult Normal: Liver
T2234149	Biochain	5 slides	EUR 185

Total Protein - Human Adult Normal Tissue: Liver
P1234149	Biochain	1 mg	EUR 188

cDNA - Human Adult Normal Tissue: Whole Eye
C1234108-10	Biochain	10 reactions	EUR 468

cDNA - Human Adult Normal Tissue: Brain: Pons
C1234071-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Eye: Retina
C1234110-10	Biochain	10 reactions	EUR 468

cDNA - Human Adult Normal Tissue: Lymph Node
C1234161-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Bone Marrow
C1234024-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Spinal Cord
C1234234	Biochain	40 reactions	EUR 385

Membrane Protein - Human Adult Normal Tissue: Liver
P3234149	Biochain	0.1 mg	EUR 270

cDNA - Human Adult Normal Tissue: Colon Sigmoid
C1234095	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Uterus: Cervix
C1234275	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Uterus: Corpus
C1234276-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Uterus: Fundus
C1234278-10	Biochain	10 reactions	EUR 215

Human Adult Liver (Normal) Whole tissue lysate
HAL-1304	Alpha Diagnostics	1 mg	EUR 628.8

cDNA - Human Adult Normal Tissue: Brain: Amygdala
C1234036-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Fallopian Tube
C1234115-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Salivary Gland
C1234212-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Stomach: Cardia
C1234250-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Stomach: Corpus
C1234251-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Stomach: Fundus
C1234252-10	Biochain	10 reactions	EUR 215

FFPE Total RNA - Human Adult Normal Tissue: Liver
R2234149	Biochain	1 ug	EUR 689

cDNA - Human Adult Normal Tissue: Brain: Pituitary
C1234068-10	Biochain	10 reactions	EUR 261

cDNA - Human Adult Normal Tissue: Colon Ascending
C1234091	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Skeletal Muscle
C1234171	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Small Intestine
C1234226	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Stomach: Pylorus
C1234253-10	Biochain	10 reactions	EUR 215

cDNA - Rat Normal Tissue: Liver
C1434149	Biochain	40 reactions	EUR 385

cDNA - Dog Normal Tissue: Liver
C1734149	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Brain: Cerebellum
C1234039	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Colon Descending
C1234092	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Colon Transverse
C1234096	Biochain	40 reactions	EUR 385

FFPE Genomic DNA - Human Adult Normal Tissue: Liver
D2234149	Biochain	2 ug	EUR 684

cDNA - Human Adult Normal Tissue: Brain: Hippocampus
C1234052-10	Biochain	10 reactions	EUR 261

cDNA - Mouse Normal Tissue: Liver
C1334149	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Blood Vessel: Vein
C1234020-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Heart: Aorta Valve
C1234123-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Heart: Atrium (left)
C1234126	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Heart: Atrium (right)
C1234127	Biochain	40 reactions	EUR 385

Total RNA - Human Adult Normal Tissue: Liver: Left Lobe
R1234150-50	Biochain	50 ug	EUR 170

cDNA - Human Adult Normal Tissue: Blood Vessel: Artery
C1234013-10	Biochain	10 reactions	EUR 215

Total RNA - Human Adult Normal Tissue: Liver: Right Lobe
R1234151-50	Biochain	50 ug	EUR 170

cDNA - Human Adult Normal Tissue: Heart: Ventricle (left)
C1234138	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Brain: Cerebellum (left)
C1234040-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Brain: Cerebral Cortex
C1234042-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Brain: Corpus Callosum
C1234045-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Heart: Ventricle (right)
C1234139	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Small Intestine: Ileum
C1234227	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Brain: Cerebellum (right)
C1234041-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Brain: Olfactory (region)
C1234063-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Brain: Vermis Cerebelli
C1234082-10	Biochain	10 reactions	EUR 215

Total Protein - Human Adult Normal Tissue: Liver: Left Lobe
P1234150	Biochain	1 mg	EUR 188

cDNA - Human Adult Normal Tissue: Small Intestine: Jejunum
C1234230	Biochain	40 reactions	EUR 385

Total Protein - Human Adult Normal Tissue: Liver: Right Lobe
P1234151	Biochain	1 mg	EUR 188

cDNA - Human Adult Normal Tissue: Small Intestine: Duodenum
C1234101-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Lung: Left Lower Lobe
C1234155	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Lung: Left Upper Lobe
C1234156	Biochain	40 reactions	EUR 385

Membrane Protein - Human Adult Normal Tissue: Liver: Left Lobe
P3234150	Biochain	0.1 mg	EUR 270

cDNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte
C1234148-10	Biochain	10 reactions	EUR 215

cDNA - Human Adult Normal Tissue: Lung: Right Lower Lobe
C1234157	Biochain	40 reactions	EUR 385

cDNA - Human Adult Normal Tissue: Lung: Right Upper Lobe
C1234159	Biochain	40 reactions	EUR 385

Total RNA - Human Adult Normal Tissue 5 Donor Pool: Liver
R1234149-P	Biochain	50 ug	EUR 370

cDNA - Human Adult Normal Tissue: Lung: Right Middle Lobe
C1234158	Biochain	40 reactions	EUR 385

cDNA - Monkey (Rhesus) Normal Tissue: Liver
C1534149	Biochain	40 reactions	EUR 385

cDNA - Monkey (Cynomolgus) Normal Tissue: Liver
C1534149-Cy	Biochain	40 reactions	EUR 385

Monkey (Cynomolgus) cDNA Normal Tissue: Liver
MC34-149	Alpha Diagnostics	10 rxn	EUR 498

Chicken (Normal/Adult) Universal (pool of tissue cDNA)
CHUC-565	Alpha Diagnostics	10 rxn	EUR 498

Genomic DNA - Human Adult Normal Tissue: Liver, from a single donor
D1234149	Biochain	100 ug	EUR 286

Total RNA - Human Adult Normal Tissue: Liver, Ambient temperature shipping
ATR1234149-50	Biochain	50 ug	EUR 185

FFPE and Frozen Matched Pair Total RNA: Human Adult Normal Tissue: Liver
R8234149-FP	Biochain	2x1 ug	EUR 1664

FFPE and Frozen Matched Pair Genomic DNA: Human Adult Normal Tissue: Liver
D8234149-FP	Biochain	2 x 2 ug	EUR 2099

Optimax First Strand cDNA Synthesis Kit
K4201100	Biochain	100 rnx	EUR 424

cDNA - Monkey (Rhesus) Normal Tissue: Liver: Left Lobe
C1534150	Biochain	40 reactions	EUR 385

cDNA - Monkey (Rhesus) Normal Tissue: Liver: Right Lobe
C1534151	Biochain	40 reactions	EUR 385

cDNA - Monkey (Cynomolgus) Normal Tissue: Liver: Left Lobe
C1534150-Cy	Biochain	40 reactions	EUR 385

cDNA - Monkey (Cynomolgus) Normal Tissue: Liver: Right Lobe
C1534151-Cy	Biochain	40 reactions	EUR 385

Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Liver
C8235149-PP	Biochain	10 reactions x2	EUR 530

igScript First Strand cDNA Synthesis Kit - 25 Reactions
4312	Intact Genomics	1/EA	EUR 178.8

igScript First Strand cDNA Synthesis Kit - 100 Reactions
4314	Intact Genomics	1/EA	EUR 453.6

Human Adult cDNA Tissue: Lung
HA-152	Alpha Diagnostics	10 rxn	EUR 498

Human Adult cDNA Tissue: Skin
HA-218	Alpha Diagnostics	10 rxn	EUR 498

Total RNA - Human Adult Normal Tissue: Lung
R1234152-50	Biochain	50 ug	EUR 170

Total RNA - Human Adult Normal Tissue: Skin
R1234218-50	Biochain	50 ug	EUR 284

Total RNA - Human Adult Normal Tissue: Brain
R1234035-50	Biochain	50 ug	EUR 170

Total RNA - Human Adult Normal Tissue: Cecum
R1234089-10	Biochain	10 ug	EUR 183

Total RNA - Human Adult Normal Tissue: Colon
R1234090-50	Biochain	50 ug	EUR 170

Total RNA - Human Adult Normal Tissue: Heart
R1234122-50	Biochain	50 ug	EUR 170

Total RNA - Human Adult Normal Tissue: Ovary
R1234183-10	Biochain	10 ug	EUR 643

Total RNA - Human Adult Normal Tissue: Penis
R1234194-50	Biochain	50 ug	EUR 170

Normal liver tissue array
LVN241	TissueArray	each	EUR 198
Description: Normal liver tissue array, 24 cases/24 cores, replaced by LVN241a

Normal liver tissue array
LVN241a	TissueArray	each	EUR 198
Description: Normal liver tissue array, 24 cases/24 cores, replacing LVN241

Human Adult cDNA Tissue: Testis
HA-260	Alpha Diagnostics	10 rxn	EUR 498

Total RNA - Human Adult Normal Tissue: Breast
R1234086-50	Biochain	50 ug	EUR 284

Total RNA - Human Adult Normal Tissue: Kidney
R1234142-50	Biochain	50 ug	EUR 170

Total RNA - Human Adult Normal Tissue: Rectum
R1234206-50	Biochain	50 ug	EUR 170

Total RNA - Human Adult Normal Tissue: Spleen
R1234246-50	Biochain	50 ug	EUR 170

Total RNA - Human Adult Normal Tissue: Testis
R1234260-50	Biochain	50 ug	EUR 170

Total RNA - Human Adult Normal Tissue: Throat
R1234263-10	Biochain	10 ug	EUR 191

Total RNA - Human Adult Normal Tissue: Thymus
R1234264-10	Biochain	10 µg	EUR 643

Total RNA - Human Adult Normal Tissue: Tongue
R1234267-50	Biochain	50 ug	EUR 170

Total RNA - Human Adult Normal Tissue: Tonsil
R1234268-10	Biochain	10 ug	EUR 191

Total RNA - Human Adult Normal Tissue: Uterus
R1234274-50	Biochain	50 ug	EUR 170

Total RNA - Human Adult Normal Tissue: Vagina
R1234283-10	Biochain	10 ug	EUR 191

Total RNA - Human Adult Normal Tissue: Adipose
R1234003-10	Biochain	10 ug	EUR 183

Total RNA - Human Adult Normal Tissue: Adrenal
R1234004-50	Biochain	50 ug	EUR 199

Total RNA - Human Adult Normal Tissue: Bladder
R1234010-50	Biochain	50 ug	EUR 170

Total RNA - Human Adult Normal Tissue: Trachea
R1234160-50	Biochain	50 ug	EUR 170

Total RNA - Human Adult Normal Tissue: Parotid
R1234190-50	Biochain	50 ug	EUR 199

Total RNA - Human Adult Normal Tissue: Stomach
R1234248-50	Biochain	50 ug	EUR 170

Total RNA - Human Adult Normal Tissue: Thyroid
R1234265-50	Biochain	50 ug	EUR 170

Frozen Tissue Array - Human Adult Normal Tissue, Multi-tissue I
T6234700-1	Biochain	1 slide	EUR 265

Frozen Tissue Array - Human Adult Normal Tissue, Multi-tissue I
T6234700-5	Biochain	5 slides	EUR 1004

Total Protein - Human Adult Normal Tissue: Lung
P1234152	Biochain	1 mg	EUR 188

Total Protein - Human Adult Normal Tissue: Skin
P1234218	Biochain	1 mg	EUR 188

Total RNA - Human Adult Normal Tissue: Appendix
R1234006-10	Biochain	10 ug	EUR 191

Total RNA - Human Adult Normal Tissue: Pancreas
R1234188-50	Biochain	50 ug	EUR 170

Total RNA - Human Adult Normal Tissue: Placenta
R1234200-50	Biochain	50 ug	EUR 170

Total RNA - Human Adult Normal Tissue: Prostate
R1234201-50	Biochain	50 ug	EUR 170

Total Protein - Human Adult Normal Tissue: Brain
P1234035	Biochain	1 mg	EUR 188

Total Protein - Human Adult Normal Tissue: Cecum
P1234089	Biochain	1 mg	EUR 188

Total Protein - Human Adult Normal Tissue: Colon
P1234090	Biochain	1 mg	EUR 188

Total Protein - Human Adult Normal Tissue: Heart
P1234122	Biochain	1 mg	EUR 188

Total Protein - Human Adult Normal Tissue: Ovary
P1234183	Biochain	1 mg	EUR 771

We found that all strains of GAS are propagated recovered from blood and spleen of mice dying have a good mutation in a gene or genes covR covS. Site mutations in the GAS strains recovered from blood and spleen identical in each mouse, while strains recovered from muscle strains including a mixture of disseminated, other mutant strains and the parent strain.